Biology concept vitamin c content in various fruit juice essay
If you know the concentration of the DCPIP the school technicians should be able to tell youyou can work backwards using the titration calculation to find out the concentration of vitamin C in the juice. Prediction Before I started the investigation I made sure I did a hypothesis so that I would be able to refer back to it in the conclusion at the end.
This would make it more accurate, as the reading would be able to be made more precisely.
Vitamin c content in different fruit juices
Dairy farm business plan example Biology coursework vitamin c This nutrient also keeps cholesterol in the bloodstream from oxidizing, another early step in the progression towards heart disease and stroke. Equation 3. Interferences due to diketogulonic acid: Due to the destructive oxidation hydrolysis at higher pH results the opening of the lactone ring of the ascorbic acid and loose the vitamin activity. This means that vitamin C is susceptible to change and unstable or that the vitamin C can break down easily if exposed to high temperatures or is kept for a long time on a shelf. The commercial orange juice is continuosly added until Dcpip solution is decolourised. Here diketogulonic acid was prepared by the acid hydrolysis dilute HNO3 of ascorbic acid. Do this on each day that you measure the vitamin C in your orange juice samples. Can you explain your results? However, there are recommended daily allowances. Introduction In this experiment I will be finding out which fruit juice contains the most vitamin C. You should use a funnel to do this. The shape of this flask allows you to swirl the solution to mix it without spilling. In your lab notebook, write down the initial level in mL of the iodine solution in the buret.
This is shown on the bar graph with the mean volume of the different types of juices needed to decolourise the DCPIP against the different types of juices. Ascorbic acid is also said to act as a detoxified and may reduce the side effects of drugs such as cortisone, aspirin, and insulin; it may also reduce the toxicity of the heavy metals lead, mercury, and arsenic, either by controlling Oxidation State or by facilitating excretion.
Investigatory project on estimation of vitamin c content in different types of fruits
Not all studies agree, however. This made the colour change more clearly visible. This means you will need to use both bottles to store the mL of solution. Vitamin C therefore aids the body in the efficient use of folic acid and iron. Another factor I would change if I were to do the investigation again is that I would use other brands of orange juices as well to widen the results I was going to get. Titrate the fresh-squeezed orange juice you just prepared by repeating step 7, but this time, use 20 mL of fresh-squeezed orange juice in the Erlenmeyer flask instead of 20 mL of the vitamin C solution. This was shown as more than 50 ml on the bar chart, but in each case 75 ml was run in without colour change observed. The juice also tasted bad.
If more than one drop is added at a time, the data will not be as accurate as it could be. The reliability of this method is also confirmed from the consideration of the following expected interferences.
Https www scribd com doc 149327779 the vitamin c content of fruit juice
Extracts from this document In this experiment, beetroots will be used to test out the aim. Introduction Vitamin C, also known as L-ascorbic acid, is a water-soluble vitamin that is naturally present in some foods, added to others, and available as a dietary supplement. On the bar chart it is shown as greater than 50 ml though. The hypothesis was that fresh fruit juices should contain more vitamin C since they had not been heat treated and probably had spent less time on a shelf or being transported than commercially sod drinks. Lastly the end point may be misjudged, as it is quite tricky to say exactly when the DCPIP has become colourless. The amount of vitamin C present in a vitamin tablet can therefore be determined by titrating a known amount of the tablet with an oxidising agent. This means there is 16 mg of vitamin C in that orange juice sample. If I was to do this investigation again I would make sure that the instructions were read clearly and also do the experiment on a larger scale, so instead of adding 5 cm3 of the DCPIP I would add 20 cm3 and therefore use a burette to add in the different types of orange juices. Many people today rely on vitamin supplement tablets.
Titrate 20 mL of vitamin C standard solution. Calculate and record the concentration of vitamin C contain in vitamin C tablet using the formula.
With vitamin C being such a useful substance to our bodies, finding good sources of vitamin C is important. Personally I chose the bar graph as it was easier to construct and also easier to understand.
How to test for vitamin c in fruit
Many people today rely on vitamin supplement tablets. Some were heavily supplemented with vitamin C e. Also the fact that there would be enough equipment would mean that I would also get the results I need, and that there can be a different piece of equipment used for each of the different experiments. Adjust the buret's position so you can place the 50 mL Erlenmeyer flask right underneath it. Figure 1. Water-soluble vitamins dissolve in water. Repeat the procedure and average result To calculate how near our value is to the actual value of vitamin C in the other orange juices. After collecting all the results and finding the means of all juices, I was able to construct a bar graph. This procedure was repeated with an orange, lemon, and a mikan tangerine. The new appreciation of the role of inflammation in atherosclerosis has further elucidated the understanding of this disease, and is providing practical. The squeezer was then cleaned using distilled water.
Titrate your other orange juice samples by repeating step 9 using the premium not-from-concentrate orange juice and the orange juice made from frozen concentrate separately.
DCPIP is a chemical compound used as a redox dye. Sailors on long sea voyages suffered very often from bleeding gums, loosened teeth, and aching joints.
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